A Method for Multiple Site-Directed Mutagenesis of More than 10 Sites


Current State of the Art:

Being able to genetically manipulate a living system is an essential tool in understanding how living systems work. Site directed mutagenesis strategies allow researchers to change DNA sequences to determine how this will influence the function of a protein or important DNA regulatory element.

Site-Directed mutagenesis strategies are used globally in almost any laboratory interested in manipulating genetics or protein function; or for engineering biological materials.

The life sciences research tools market was estimated at $42 billion in 2010. Reagents and tools for DNA and RNA research are currently worth $27 billion and expected to increase at a 12.2% compound annual growth rate (CAGR) by 2016.


Disadvantages with the Current Art:

Available methods are limited in their ability to mutate multiple sites within DNA effectively and efficiently. This situation forces researchers to use non-standardized methods to produce the resources they need. For example, complex mutagenesis schemes, like those for the identification of important phosphorylation sites, can create artifacts where non-optimal phosphorylation sites will be used ad hoc in vivo to compensate for loss of the optimal phosphorylation site targeted for mutagenesis. To compensate for this situation researchers often employ many steps, requiring more time and reagents, in order to mutate multiple sites.


Advantages of Invention:

Dr. Seyfang has developed and optimized a method for site directed mutagenesis that would be an invaluable tool for molecular biology, protein biochemistry and genetic engineering.

·         Advantages of a kit based on this patented invention process:

·         Simultaneous mutation of up to 10 sites

·         Single round of synthesis

·         100% efficiency

·         Inexpensive


Patent Status: Patent Issued 6,878,531


Inventor: Andreas Seyfang, PhD

Case Number: GHSU 2004-002



Patent Information:
Research Tools
For Information, Contact:
Augusta University
Andreas Seyfang
Genetic Engineering
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